Transferrin receptor, BioLegend, 334102

We here share a literature review of commercially available transferrin receptor antibodies validated in the Caco-2 cells in vitro transcytosis model by Deng et al. from AbbVie, published in PLoS One 2023. The authors screen a panel of thirteen commercial human transferrin receptor antibodies, rank their binding potency to human transferrin receptor overexpressed in BaF3 cells, test their cross reactivity to cyno and mouse transferrin receptor, and their transcytosis in Caco-2 cells compared to control IgG.
According to the table in Figure 2D, clone CY1G4 shows 3.2 fold transcytosis over control IgG despite high affinity for the transferrin receptor in transfected cells (EC50 = 0.08 nM).
CY1G4 does not cross react with monkey or mouse transferrin receptor.
We highly encourage other scientists to share their experiences.