ADNP, Abcam, ab300114

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human ADNP and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 7 commercial research antibodies against human ADNP.

The collection of antibodies was tested using DMS 53 WT and ADNP KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ab300114 detected a single band of appr. 135 kDa in WT lysate, with no band observed in KO lysate. In the following IP (2 µg Ab used) several stronger background bands of lower molecular weight were seen compared to input extract (6% of starting material), furthermore no band was observed in the unbound fraction. In IF (1:500 Ab dilution), a clear intense staining was observed in WT cells with no background staining in KO cells. We highly encourage other scientists to share their observations.