Annexin A11, GeneTex, GTX33010

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Annexin A11 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 15 commercial research antibodies against human Annexin A11 encoded by ANAX11.

The collection of antibodies was tested using HeLa WT and ANAX11 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) GTX33010 detected no band for either WT or KO cell lysate. No band was detected following IP (1 µg Ab used). In IF (1:800 Ab dilution), weaker staining was observed in both PFA and methanol fixation for KO compared to WT. We highly encourage other scientists to share their observations.