Annexin A11, Thermo Fisher Scientific, PA5-82983

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Annexin A11 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 15 commercial research antibodies against human Annexin A11 encoded by ANAX11.

The collection of antibodies was tested using HeLa WT and ANAX11 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) PA5-82983 detected a band of appr. 54 kDa in WT lysate not observed in KO lysate. Several background bands of both higher and lower molecular weight were seen in WT and KO lysates. A band was also detected following IP (1 µg Ab used). This band was as intense than the corresponding band in input (10% of starting material). Also, the antibody did not deplete the antigen from the cell lysate. In IF (1:300 Ab dilution) comparable staining intensities were observed in both KO and WT cells. We highly encourage other scientists to share their observations