Annexin A11, Thermo Fisher Scientific, MA5-25052

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Annexin A11 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 15 commercial research antibodies against human Annexin A11 encoded by ANAX11.

The collection of antibodies was tested using HeLa WT and ANAX11 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution) MA5-25052 detected a band of appr. 54 kDa in WT lysate, with no corresponding band observed in KO lysate. A low molecular weight band was detected in lysates of both genotypes. A band was also detected following IP (1 µg Ab used). This band was more intense than the corresponding band in input extract (10% of starting material). No band was detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:1000 Ab dilution), for both PFA and methanol fixation, a weaker staining of KO cells was observed compared to KO cells. We highly encourage other scientists to share their observations.