CD44, Abcam, ab101531

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human CD44 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 32 commercial research antibodies against human CD44.

The collection of antibodies was tested using MDA-MB-231 WT and CD44 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IF (WT and KO cells) and FC (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ab101531 detected a single band of appr. 81 kDa in WT lysate, with no corresponding band observed in KO lysate. In IF (1:50 Ab dilution), a clear intense staining was observed in WT cells with no background staining in KO cells. Cell surface flow cytometry (1 µg/mL antibody) showed a clear fluorescence shift in the WT cell population compared with the KO cells, indicating specific binding of the antibody to the target protein. Intracellular flow cytometry showed some background for KO cells.

We highly encourage other scientists to share their observations.