Cyclin-F, Cell Signaling Technology, 81045

O+H4ur team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Cyclin-F and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing three commercial research antibodies against human Cyclin-F encoded by CCNF.

The collection of antibodies was tested using U2OS WT and CCNF KO cells in the applications WB (WT and KO cell lysates/30 µg protein), according to YCharOS standard protocols (see reference).

U2OS remained untreated (-) or were synchronized in the G2 phase by a double thymidine block followed by an 8 hours release into complete medium (+). In WB for thymidin treated cells (1:1000 Ab dilution) 81045 detected a single band of appr. 87 kDa for WT lysate, with no corresponding band in KO lysate.

We highly encourage other scientists to share their observations.