Calpain-2 catalytic subunit, Cell Signaling Technology, 2539

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Calpain-2 catalytic subunit and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Calpain-2 catalytic subunit encoded by CAPN2.

The collection of antibodies was tested using MDA-MB-231 WT and CAPN2 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) 2539 detected a single band of appr. 74 kDa in WT lysate, with no band observed in KO lysate. No band was observed in the following IP (1 µg Ab used). In IF (1:500 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.