Calpain-2 catalytic subunit, Abcam, ab39165

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Calpain-2 catalytic subunit and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Calpain-2 catalytic subunit encoded by CAPN2.

The collection of antibodies was tested using MDA-MB-231 WT and CAPN2 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 Ab dilution) ab39165 detected a single band of appr. 74 kDa in WT lysate, with no band observed in KO lysate. A band of higher molecular weight was observed in both genotypes. No band was observed in the following IP (1 µg Ab used). ). In IF (1:1000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.