CaMK-II subunit delta, Thermo Fisher Scientific, MA5-32680

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human CaMK-II subunit delta and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human CaMK-II subunit delta encoded by CAMK2D.

The collection of antibodies was tested using HAP1 WT and CAMK2D KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-32680 detected a single band of appr. 56 kDa in WT lysate, with no band observed in KO lysate. No band was observed in the following IP (2 µg Ab used) for the predicted antigen, moreover a band for heavy chain was observed. In IF (1:500 Ab dilution), a weak staining of WT cells was observed compared to KO cells.

We highly encourage other scientists to share their observations.