CaMK-II subunit delta, Bio-Techne, MAB4176

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human CaMK-II subunit delta and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human CaMK-II subunit delta encoded by CAMK2D.

The collection of antibodies was tested using HAP1 WT and CAMK2D KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) MAB4176 detected two intense bands for both genotypes, but none for the protein of interest. No band was observed in the following IP (2 µg Ab used) for the predicted antigen. In IF (1:500 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.