Ataxin-3, Aviva Systems Biology, OAAB05835

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against humanAtaxin-3 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing seven commercial research antibodies against human Ataxin-3 encoded by ATXN3

The collection of antibodies was tested using HEK293T WT and ATXN3 KO cells in WB (WT and KO cell lysates/30 µg protein), IP (HEK293T WT cell lysate) and IF (U2OS WT and ATXN3 KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) OAAB05835 detected no band of appr. 42 kDa in WT or KO cell lysate. Several weaker background bands of both higher and lower molecular weight seen in WT and KO lysates. Only a very weak band was detected following IP (1 µg Ab used). Bands were also detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does not deplete the antigen from the cell lysate. In IF (1:500 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.