Ataxin-3, Abcam, ab96316

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Ataxin-3 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing seven commercial research antibodies against human Ataxin-3 encoded by ATXN3

The collection of antibodies was tested using HEK293T WT and ATXN3 KO cells in WB (WT and KO cell lysates/30 µg protein), IP (HEK293T WT cell lysate) and IF (U2OS WT and ATXN3 KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) ab96316 produces multiple bands of both higher and lower molecular weight. A band of appr. 42 kDa was observed in both WT and KO cell lysates. . A double band was also detected following IP (1 µg Ab used). This double band was marked more intense than the corresponding band in input extract (10 % of starting material), Furthermore, no band was observed in immunodepleted extract (10% of total unbound fraction) showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:400 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.