Ataxin-3, Thermo Fisher Scientific, 702788

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Ataxin-3 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing seven commercial research antibodies against human Ataxin-3 encoded by ATXN3

The collection of antibodies was tested using HEK293T WT and ATXN3 KO cells in WB (WT and KO cell lysates/30 µg protein), IP (HEK293T WT cell lysate) and IF (U2OS WT and ATXN3 KO cells) according to YCharOS standard protocols (see reference).

In WB (1:50 Ab dilution) 702788 detected a double band of appr. 42 kDa in WT cell lysate, with no band observed in KO cell lysate. A double band was also detected following IP (1 µg Ab used). This double band was marked more intense than the corresponding band in input extract (10 % of starting material), Furthermore, no band was observed in immunodepleted extract (10% of total unbound fraction) showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:500 ab dilution) WT cells had more intense staining compared to KO cells, which had background staining

We highly encourage other scientists to share their observations.