Ataxin-2, GeneTex, GTX130331

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Ataxin-2 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing five commercial research antibodies against human Ataxin-2 encoded by ATXN2.

The collection of antibodies was tested using HAP1 WT and ATXN2 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) GTX130331 produces multiple bands, none of which were specific to WT or KO lysates. No band was detected following IP (1 µg Ab used). In IF (1:1000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.