Plasma membrane calcium-transporting ATPase 1, Thermo Fisher Scientific, PA1-914

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Plasma membrane calcium-transporting ATPase 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing five commercial research antibodies against human Plasma membrane calcium-transporting ATPase 1 encoded by ATP2B1.

The collection of antibodies was tested using HAP1 WT and ATP2B1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) PA1-914 detected no band for either WT or KO cell lysates. No band was detected following IP (2 µg Ab used). In IF (1:200 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.