Rho GDP-dissociation inhibitor 1, Thermo Fisher Scientific, MA5-25081

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rho GDP-dissociation inhibitor 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Rho GDP-dissociation inhibitor 1 encoded by ARHGDIA.

The collection of antibodies was tested using HEK-293T and ARHGDIA KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-25081 detected a band of appr. 23 kDa in WT lysate, with no corresponding band observed in KO lysate. No band was detected following IP (2 µg Ab used). In IF a weak staining of WT cells was observed compared to KO cells.

We highly encourage other scientists to share their observations.