Rho GDP-dissociation inhibitor 1, Abcam, ab135252

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rho GDP-dissociation inhibitor 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Rho GDP-dissociation inhibitor 1 encoded by ARHGDIA.

The collection of antibodies was tested using HEK-293T and ARHGDIA KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ab135252 detected a band of appr. 23 kDa in WT lysate, with no corresponding band observed in KO lysate. One band of higher molecular weight were detected in both WT and KO cell lysates. No band was detected following IP (2 µg Ab used). In IF comparable staining intensities were observed in both KO and WT cells, with exception of a few WT cells showing more intense staining.

We highly encourage other scientists to share their observations.