ApoE, Bio-Techne, NB110-60531

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Apolipoprotein E and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Apolipoprotein E encoded by APOE.

The collection of antibodies was tested using HAP1 WT and APOE KO cells in the applications WB (WT and KO culture media/30 µg protein) and IP (WT culture media) according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution) NB110-60531 detected no band of appr. 36 kDa in WT or KO culture media. Several bands of higher molecular weight were observed for both WT and KO culture media. A band was also detected following IP (2 µg Ab used), but band intensity was comparable to input extract ( 3% of starting material). Band was also detected in immunodepleted extract (3% of total unbound fraction), showing that the antibody does not deplete the antigen from the culture media.

We highly encourage other scientists to share their observations.

WUE-4 antibody against Apolipoprotein E works very well to capture ApoE particles in cell media for ELISA experiments.
The concentration used was 1µg/mL.