ApoE, Cell Signaling Technology, 13366

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Apolipoprotein E and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Apolipoprotein E encoded by APOE.

The collection of antibodies was tested using HAP1 WT and APOE KO cells in the applications WB (WT and KO culture media/30 µg protein) and IP (WT culture media) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) 13366 detected a band of appr. 36 kDa in WT culture media, with no corresponding band observed in KO culture media. Several bands of higher molecular weight were observed in WT culture media. A band was also detected following IP (2 µg Ab used). This band was markedly more intense than the barely detectable band in input extract (3% of starting material).

We highly encourage other scientists to share their observations.