ACE, Thermo Fisher Scientific, PA5-86568

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Angiotensin-converting enzyme and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 15 commercial research antibodies against human Angiotensin-converting enzyme encoded by ACE.

The collection of antibodies was tested using SK-N-FI WT and ACE KO cells in the applications WB (WT and KO cell lysates/40 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) PA5-86568 detected a band of appr. 150 kDa in WT lysate, with a less intense stained band in KO lysate. Furthermore, several bands of lower molecular weight were seen for both genotypes. No band was detected following IP (2 µg Ab used)

We highly encourage other scientists to share their observations.