ACE, Abcam, ab254222

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Angiotensin-converting enzyme and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 15 commercial research antibodies against human Angiotensin-converting enzyme encoded by ACE.

The collection of antibodies was tested using SK-N-FI WT and ACE KO cells in the applications WB (WT and KO cell lysates/40 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference). Furthermore, this antibody was tested in HAP1 WT by Western blot.

In WB (1:10000 Ab dilution) ab254222 detected a band of appr. 150 kDa in WT lysate, with a corresponding less intense stained band in KO lysate. An additional WB was carried out in SK-N-FI WT and KO cell lysate and HAP1 WT lysate. In this WB (1:10000 Ab dilution) ab254222 detected band of equal intensity in SK-N-FI KO and WT cell lysate. A band of lower intensity was also detected in HAP1 WT. No band was detected following IP (2 µg Ab used)

We highly encourage other scientists to share their observations.