ACSL5, Thermo Fisher Scientific, PA5-52392

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human ACSL5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human ACSL5.

The collection of antibodies was tested using HCT 116 WT and ACSL5 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) PA5-52392 detected a band of appr. 63 kDa in WT lysate, with no corresponding band observed in KO lysate. One weaker background band of higher molecular weight are seen in both WT and KO. In the following IP (2 µg Ab used) a faint band was observed.
In IF (1:100 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.