ACSL5, Proteintech, 15708-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human ACSL5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human ACSL5.

The collection of antibodies was tested using HCT 116 WT and ACSL5 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) 15708-1-AP detected a band of appr. 63 kDa in WT lysate, with no corresponding band observed in KO lysate. A faint high molecular weight band detected in lysates of both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:150 Ab dilution), a slightly more intense staining was observed in WT cells with some background staining in KO cells.

We highly encourage other scientists to share their observations.