ACSL5, Bio-Techne, NB100-2918

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human ACSL5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human ACSL5.

The collection of antibodies was tested using HCT 116 WT and ACSL5 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) NB100-2918 detected a band of appr. 63 kDa in WT lysate, with no corresponding band observed in KO lysate. One weaker background band of higher molecular weight are seen in WT and bands of both higher and lower molecular weight are seen in KO lysates. No band was detected following IP (2 µg Ab used). In IF (1:500 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.