ACSL5, Abcam, ab188507

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human ACSL5 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human ACSL5.

The collection of antibodies was tested using HCT 116 WT and ACSL5 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ab188507 detected a band of appr. 63 kDa in WT lysate not observed in KO lysate. Several weaker background bands of both higher and lower molecular weight seen in WT and KO lysates.
A band was also detected following IP (2 µg Ab used). For western blot in IP, MA5-24595 was used at 1:200. This band was markedly more intense than the corresponding band in input extract (4% of starting material). Band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:150 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.