TYRO protein tyrosine kinase-binding protein, Thermo Fisher Scientific, PA5-26861

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human TYRO protein tyrosine kinase-binding protein, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 9 commercially available antibodies against TYRO protein tyrosine kinase-binding protein across multiple applications.

Antibodies were tested by WB using lysates (100 µg protein) from THP-1 WT (ab271147) and TYRO protein tyrosine kinase-binding protein deficient (TYROBP KO) (ab273726) cells. These cell lines were also used for IF. In addition, antibodies were evaluated by IP using THP-1 WT cell lysate (1 mg protein). All experiments were performed according to YCharOS standard protocols (see reference).

In WB (1:1000 antibody dilution), PA5-26861 detected a weak band in the THP-1 WT lysate below the 11 kDa marker, with no corresponding band in TYROBP KO cells, supporting specificity. Although this is lower than the expected size (~12 kDa), the signal is consistent with the expected band when compared across antibodies. Additionally, multiple intense bands were observed in both lysates.

In IP, the antibody (1 µg) enriched TYRO protein tyrosine kinase-binding protein upon precipitation, with no detectable target protein remaining in the unbound fraction. TYRO protein tyrosine kinase-binding protein levels in the starting material, unbound fraction, and precipitate were assessed by WB using the TYRO protein tyrosine kinase-binding protein-specific antibody 12492 from Cell Signaling Technology (1:1000).

In IF, the antibody (1:400 dilution) failed to produce a TYRO protein tyrosine kinase-binding protein-specific staining in THP-1 WT cells.

We highly encourage other scientists to share their observations.