TYRO protein tyrosine kinase-binding protein, Thermo Fisher Scientific, MA5-34787

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human TYRO protein tyrosine kinase-binding protein, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 9 commercially available antibodies against TYRO protein tyrosine kinase-binding protein across multiple applications.

Antibodies were tested by WB using lysates (100 µg protein) from THP-1 WT (ab271147) and TYRO protein tyrosine kinase-binding protein deficient (TYROBP KO) (ab273726) cells. These cell lines were also used for IF. In addition, antibodies were evaluated by IP using THP-1 WT cell lysate (1 mg protein). All experiments were performed according to YCharOS standard protocols (see reference).

In WB (1:500 antibody dilution), MA5-34787 did not detect a clear band at the expected size (~12 kDa). Instead, a double band at higher molecular weight (~90-100 kDa) was observed in both WT and KO lysates, indicating non-specific binding.

In IP, the antibody (1 µg) failed to deplete TYRO protein tyrosine kinase-binding protein from THP-1 lysate as assessed by WB using the TYRO protein tyrosine kinase-binding protein-specific antibody 12492 from Cell Signaling Technology (1:1000).

In IF, the antibody (1:1000 dilution) produced a weak TYRO protein tyrosine kinase-binding protein-specific staining distributed throughout THP-1 WT cells, with some background in TYROBP KO cells

We highly encourage other scientists to share their observations.