TYRO protein tyrosine kinase-binding protein, Abcam, ab93846

Our team performed a comprehensive review of the scientific literature to identify studies using antibodies against human TYRO protein tyrosine kinase-binding protein, followed by extraction and evaluation of available data according to pAbmAbs rating criteria.

YCharOS conducted a systematic head-to-head comparison of 9 commercially available antibodies against TYRO protein tyrosine kinase-binding protein across multiple applications.

Antibodies were tested by WB using lysates (100 µg protein) from THP-1 WT (ab271147) and TYRO protein tyrosine kinase-binding protein deficient (TYROBP KO) (ab273726) cells. These cell lines were also used for IF. In addition, antibodies were evaluated by IP using THP-1 WT cell lysate (1 mg protein). All experiments were performed according to YCharOS standard protocols (see reference).

In WB (1:1000 antibody dilution), ab93846 detected a clear band in the THP-1 WT lysate below the 11 kDa marker, with no corresponding band in TYROBP KO cells, supporting specificity. Although this is lower than the expected size (~12 kDa), the signal is consistent with the expected band when compared across antibodies. Additionally, multiple intense bands above 25 kDa were observed in both lysates.

In IP, the antibody (1 µg) failed to deplete TYRO protein tyrosine kinase-binding protein from THP-1 lysate as assessed by WB using the TYRO protein tyrosine kinase-binding protein-specific antibody 12492 from Cell Signaling Technology (1:1000).

In IF, the antibody (1:1000 dilution) produced a weak TYRO protein tyrosine kinase-binding protein-specific staining in THP-1 WT cells, with some background in TYROBP KO cells

We highly encourage other scientists to share their observations.