Neurosecretory protein VGF, Abcam, ab74140

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human neurosecretory protein VGF and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.
YCharOS is here conducting a large head-to-head study comparing 7 commercial research antibodies against human VGF.
The collection of antibodies was tested using U2OS WT and Vgf KO cells in the applications WB and IP according to YCharOS standard protocols (see reference). WB was performed using lysates (100 µg protein) from WT and KO cells with and without Brefeldin A treatment and cell culture supernatant. IP was performed using WT and KO cell culture supernatant. Western blotting with ab74140 (antibody dilution 1:500) did not detect any proteins in cell culture supernatant, and a similar pattern with > 10 bands was observed for lysates from WT and KO cells. Additionally, the antibody (1 µg) failed to immunoprecipitate the antigen from WT cell culture supernatant. We highly encourage other scientists to share their observations.