VPS35, Thermo Fisher Scientific, MA5-34647

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human VPS35 and followed up extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human VPS35.

The collection of antibodies was tested using HAP1 WT and VPS35 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-34647 detected a band of appr. 75 kDa in WT lysate, with no corresponding band observed in KO lysate. A faint high molecular weight band detected in lysates of both genotypes. Only a very weak band was detected following IP (2 µg Ab used). In IF (1:1000Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.