VPS35, GeneTex, GTX116260

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human VPS35 and followed up extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human VPS35.

The collection of antibodies was tested using HAP1 WT and VPS35 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) GTX116260 detected a band of appr. 75 kDa in WT lysate not observed in KO lysate. Several weaker background bands of both higher and lower molecular weight seen in WT and KO lysates. No band was detected following IP (2 µg Ab used). In IF (1:600 Ab dilution), an intense staining of WT cells was observed compared to KO cell staining.

We highly encourage other scientists to share their observations.