SGLT2, Proteintech Group, 24654-1-AP
What do reviewers generally think about 24654-1-AP from Proteintech?
Researchers report that SGLT2 antibody 24654-1-AP gives specific immunostaining in wild-type mouse and rat kidney sections, absent in Sglt2 knockout tissue, and detects an expected-size band by western blot in mouse and rat kidney homogenates without unspecific knockout bands. One reviewer points out that they struggle with western blotting despite good immunostaining, while another notes high background signal in both species. Overall, it is reported as useful for immunostaining and western blotting, though with noted background and WB difficulty.
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User Reviews (2)
Review of: SGLT2, Proteintech Group, 24654-1-AP by pAbmAbs Data Mining
We share a literature review of commercially available SGLT2 antibodies evaluated by immunohistochemistry and western blotting using kidneys and hearts from WT and SGLT2 deficient mice and rats. Antibody specificity was also evaluated in human kidney (Hirose et al., Hypertension, 2026). The authors perform a head-to-head screen of a panel of eight commercial SGLT2 antibodies.
We have rated the antibody performance according to the pAbmAbs criteria, and we highly encourage other scientists to share their experiences.
The antibody 24654-1-AP showed specific immunostaining in kidney sections from WT mice and rats (Figure 1) not observed in Sglt2-/- sections from mouse and rat. However, a high background signal was observed in both species.
Western blotting with this antibody detected a distinct band of the expected size using mouse and rat kidney homogenates as samples. No unspecific bands were observed in Sglt2-/- samples (Figure 4).
0 useful
0 not usefulReview of: User contributed: SGLT2, Proteintech Group, 24654-1-AP by Michael Schou
Works well with immunostaining. I struggle with WB.
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