This was used for western blot on tissue from mouse hippocampus (50ug protein pr sample). This works excellent for detecting mouse sortilin! Used at a dilution of 1:1000.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 22, 2015Maj Ulrichsen wrote:
5
This antibody is very sensitive in western blotting against sortilin in primary rat Schwann cell lysates. Dilution 1:1000.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On August 6, 2013Camilla Gustafsen wrote:
5
My favourite sortilin antibody for Western blotting: excellent for detection of both human and mouse sortilin in tissue homogenates and cell lysates. I dilute 1:2000 and re-use several times (∼5).
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 16, 2012Anja A wrote:
5
Works fine for detecting sortilin in all samples tested including tissues and cell lines. Dilution 1:1000 in Western blotting.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 5, 2012Mette R, Aarhus wrote:
4
Dilute 1:1000 for Western blotting.
Detects sortilin in 25 ug of mouse spinal cord lysate.
Diluted antibody can be reused at least four times
On August 19, 2015 Simon Mølgaard wrote:
This was used for western blot on tissue from mouse hippocampus (50ug protein pr sample). This works excellent for detecting mouse sortilin! Used at a dilution of 1:1000.
On May 22, 2015 Maj Ulrichsen wrote:
This antibody is very sensitive in western blotting against sortilin in primary rat Schwann cell lysates. Dilution 1:1000.
On August 6, 2013 Camilla Gustafsen wrote:
My favourite sortilin antibody for Western blotting: excellent for detection of both human and mouse sortilin in tissue homogenates and cell lysates. I dilute 1:2000 and re-use several times (∼5).
On February 16, 2012 Anja A wrote:
Works fine for detecting sortilin in all samples tested including tissues and cell lines. Dilution 1:1000 in Western blotting.
On February 5, 2012 Mette R, Aarhus wrote:
Dilute 1:1000 for Western blotting.
Detects sortilin in 25 ug of mouse spinal cord lysate.
Diluted antibody can be reused at least four times