Does not work in immunostaining cultured hippocampal neurons.
Dilution 1:200, fixed in 4 % PFA 20 min
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 25, 2013Peter Lund Ovesen wrote:
4
We observed goood results for Western blotting using membrane preperation of 20 ug mouse hippocampal samples in a dilution of 1:5000. The lower antibody concentration reduced the unspecific binding, showing only the NR2B-band.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 12, 2013Simon Glerup wrote:
4
Excellent for Western blotting using membrane preparations of mouse hippocampus as samples.
Dilution 1:1000
On January 10, 2014 Peter Lund Ovesen wrote:
Does not work in immunostaining cultured hippocampal neurons.
Dilution 1:200, fixed in 4 % PFA 20 min
On January 25, 2013 Peter Lund Ovesen wrote:
We observed goood results for Western blotting using membrane preperation of 20 ug mouse hippocampal samples in a dilution of 1:5000. The lower antibody concentration reduced the unspecific binding, showing only the NR2B-band.
On January 12, 2013 Simon Glerup wrote:
Excellent for Western blotting using membrane preparations of mouse hippocampus as samples.
Dilution 1:1000