Related topics: Alphabetical list, Hydrolases
On June 29, 2020
Michael J. Lukey, Cold Spring Harbor Laboratory wrote:
Used for western blotting of breast cancer cells. Dilution: 1:8000
Many off-target bands are stronger than the GLS2 signals. But, unlike most other GLS2 antibodies that we tested, this one does at least give a clear band for GLS2. Recommend performing knockdowns to confirm which of the many bands is GLS2.
For more information see Lukey et al. Cell Reports 2019, “Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer”.