LIVE/DEAD Fixable Near-IR Dead Cell Stain, Thermo Fisher Scientific, L10119

  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • 55555
  • No Ratings
  • No Ratings

Related topics:

One User Review

  • Amine reactive dye that bind free amines both in the interior of cells and on surface of cells with compromised cell surfaces. Dye is excited at 633 nm or 635 nm so I use APC-Cy7 channel to detect dead cells along with CD3+ cells in a multi color flow cytometry panel to stain PBMCs and expanded T cells. It works really well in both phenotyping and intracellular cytokine staining (CD Ab from BD Horizon #563800).

    Live/dead dye is diluted 1:80 in PBS supplemented with 2 % FBS and cells are then stained with 10 uL of the diluted Live/dead sulution for 20 minutes.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry55555
    LuminexNot Rated
    EMNot Rated

Leave a review

Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM