On July 6, 2020Jamie Rose, University of Edinburgh wrote:
3
Used for IF. Works at 1:200 (5ug/mL). Sometimes stains the peri-nuclear zone rather than the nucleus, though this might be due to the sample rather than the antibody.
On June 30, 2020Nicola Micali, Yale University wrote:
5
Great antibody for IF. Concentration indicated by manufacturer’s protocol.
Great antibody for IHC on monkey tissue. Dilution: 1:750
For more information see Micali et al. Cell Reports 2020, “Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates”. https://www.sciencedirect.com/science/article/pii/S2211124720305489
On July 6, 2020 Jamie Rose, University of Edinburgh wrote:
Used for IF. Works at 1:200 (5ug/mL). Sometimes stains the peri-nuclear zone rather than the nucleus, though this might be due to the sample rather than the antibody.
For more information see Pickett et al. Cell Reports 2019, “Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer’s Disease”.
https://www.sciencedirect.com/science/article/pii/S2211124719315268?dgcid=rss_sd_all#!
On June 30, 2020 Nicola Micali, Yale University wrote:
Great antibody for IF. Concentration indicated by manufacturer’s protocol.
Great antibody for IHC on monkey tissue. Dilution: 1:750
For more information see Micali et al. Cell Reports 2020, “Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates”.
https://www.sciencedirect.com/science/article/pii/S2211124720305489
On June 10, 2020 Jeannette Nardelli, Université de Paris, NeuroDiderot wrote:
Works roughly for IF on frozen sections. Dilution used: 1:500
For more information see Journiac et al. Cell Reports 2020, “ Cell Metabolic Alterations due to Mcph1 Mutation in Microcephaly”.
https://www.sciencedirect.com/science/article/pii/S221112472030396X