On November 5, 2015Camilla Gustafsen, Aarhus University wrote:
4
We have used this antibody for WB detection of LDLR in mouse liver after iv administration of PCSK9. The antibody detects a band of expected size (100 kDa), which is down-regulated in animals injected with PCSK9. The antibody also detects a band above the 188 kDa marker band (Western blot shown). 40 µg protein (membrane preparation) was loaded, and the antibody was diluted 1:1000 in 5% milk in TBS-T.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 30, 2014Simon Glerup, Aarhus University wrote:
1
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 29, 2012Simon Glerup, Aarhus wrote:
2.5
We use 1:2000 diluted for Western blotting of lysates of 293 and HepG2 cells, and liver and brain homogenates (mouse). Detects a band of the expected size.
It stains 293 cells when used in immunofluorescense but I´m not sure that it is specific.
On November 5, 2015 Camilla Gustafsen, Aarhus University wrote:
We have used this antibody for WB detection of LDLR in mouse liver after iv administration of PCSK9. The antibody detects a band of expected size (100 kDa), which is down-regulated in animals injected with PCSK9. The antibody also detects a band above the 188 kDa marker band (Western blot shown). 40 µg protein (membrane preparation) was loaded, and the antibody was diluted 1:1000 in 5% milk in TBS-T.
On October 30, 2014 Simon Glerup, Aarhus University wrote:
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
On February 29, 2012 Simon Glerup, Aarhus wrote:
We use 1:2000 diluted for Western blotting of lysates of 293 and HepG2 cells, and liver and brain homogenates (mouse). Detects a band of the expected size.
It stains 293 cells when used in immunofluorescense but I´m not sure that it is specific.