Cleaved Drosophila Dcp-1 (Asp216), rabbit pAb, Cell Signaling, 9578

  • No Ratings
  • 4.64.64.64.64.6
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings

Related topics: , , ,

5 User Reviews

  • Used for immunostaining of drosophila wing discs. 30 minutes fixation in 4% formaldehyde at room temp. Great result.
    Dilution used: 1:500

    For more information see Zappia et al. EMBO Reports 2020, “A cell atlas of adult muscle precursors uncovers early events in fibre‐type divergence in Drosophila”.
    https://www.embopress.org/doi/full/10.15252/embr.201949555

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • I see some unspecific signals when I use it with UAS-p35 flies, which may be caused by some unlucky sequence similarity with “amino-terminal residues adjacent to Asp216”. Other than that we use it frequently, seems to be working fine with IHC on L3 wing discs.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Works well for ICC on fly eye discs. Dilution: 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Works great for ICC on fly larval imaginal discs. Dilution used: 1:500

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • It took us a bit of time to make it work because there was a bit of background . Once we found the conditions it was quite good.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

Leave a review

Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM