I see some unspecific signals when I use it with UAS-p35 flies, which may be caused by some unlucky sequence similarity with “amino-terminal residues adjacent to Asp216”. Other than that we use it frequently, seems to be working fine with IHC on L3 wing discs.
On January 9, 2021 Maria Paula Zappia, University of Illinois at Chicago wrote:
Used for immunostaining of drosophila wing discs. 30 minutes fixation in 4% formaldehyde at room temp. Great result.
Dilution used: 1:500
For more information see Zappia et al. EMBO Reports 2020, “A cell atlas of adult muscle precursors uncovers early events in fibre‐type divergence in Drosophila”.
https://www.embopress.org/doi/full/10.15252/embr.201949555
On June 25, 2020 Ugurcan Sakizli wrote:
I see some unspecific signals when I use it with UAS-p35 flies, which may be caused by some unlucky sequence similarity with “amino-terminal residues adjacent to Asp216”. Other than that we use it frequently, seems to be working fine with IHC on L3 wing discs.
On June 16, 2020 Bing Zhang initiative wrote:
Works well for ICC on fly eye discs. Dilution: 1:100
On June 16, 2020 Bing Zhang initiative wrote:
Works great for ICC on fly larval imaginal discs. Dilution used: 1:500
On May 15, 2020 Jimena Berni, University of Cambridge wrote:
It took us a bit of time to make it work because there was a bit of background . Once we found the conditions it was quite good.