Somatostatin-14, rabbit pAb, Peninsula Laboratories, T4103

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One User Review

  • Immunostaining were done on 4% paraformaldehyde perfusion-fixed brains that were cut at 50µm on a vibratome.
    Antibody specificity appears fine and is consistent with literature. We used 1:1000 concentration. Some cells are incredibly well stained and others are only moderately stained, requiring you to make a inclusion criteria for counts. Background signal can be high -which can be challenging to optimize reaction.

    Signal is improved following antigen retrieval (ph 6.0 sodium citrate buffer @ 85-95 celsisus for 30 minutes).

    For more information see Botterill et al. Cell Reports 2019, “An Excitatory and Epileptogenic Effect of Dentate Gyrus Mossy Cells in a Mouse Model of Epilepsy”

    Western blotNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
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