I have used this antibody for western blotting of tissue lysates from hippocampus of C57BL/6J mice in a dilution of 1:3000. This antibody works so well in our lab. We often use it in our western blotting as a loading control for protein normalization.
On July 12, 2020 Shirin Hosseini, Technische Universität Braunschweig wrote:
I have used this antibody for western blotting of tissue lysates from hippocampus of C57BL/6J mice in a dilution of 1:3000. This antibody works so well in our lab. We often use it in our western blotting as a loading control for protein normalization.
For more information see Hosseini et al. Cell Reports 2020, “Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS”.
https://www.sciencedirect.com/science/article/pii/S2211124720306197
On April 20, 2020 Lea Friker, University of Bonn wrote:
This antibody worked great for WB of primary mouse microglia lysates.
Dilution used: 1:1000
For more information see Friker et al. Cell Reports 2020, “β-Amyloid Clustering around ASC Fibrils Boosts Its Toxicity in Microglia”.
https://www.sciencedirect.com/science/article/pii/S2211124720301868