This antibody worked fine for IF.
It requires antigen retrieval – slide-mounted cryosections were permeabilized with 0.3% Triton X-100 and subjected to antigen retrieval (Antigen retrieval buffer, Wako Chemicals) for 10–30 min, at pH 6.0, at 90°C-95°C.
Dilution used 1:500
On April 13, 2020 Gregory Kirschen, Renaissance School of Medicine at Stony Brook University wrote:
This antibody worked fine for IF.
It requires antigen retrieval – slide-mounted cryosections were permeabilized with 0.3% Triton X-100 and subjected to antigen retrieval (Antigen retrieval buffer, Wako Chemicals) for 10–30 min, at pH 6.0, at 90°C-95°C.
Dilution used 1:500
For more information see Yang et al. Cell Reports 2020, “Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain”.
https://www.sciencedirect.com/science/article/pii/S2211124719317498