Excellent staining however penetration could be an issue in thick sections (>50 micrometer). I recommend long incubations (over 3 days) in a cold room.
Dilution used: 1:1500
For more information see Ozdemir et al. Cell Reports 2020, “Unexpected Rule-Changes in a Working Memory Task Shape the Firing of Histologically Identified Delay-Tuned Neurons in the Prefrontal Cortex”
On March 31, 2020 Tugrul Ozdemir, University of Oxford wrote:
Excellent staining however penetration could be an issue in thick sections (>50 micrometer). I recommend long incubations (over 3 days) in a cold room.
Dilution used: 1:1500
For more information see Ozdemir et al. Cell Reports 2020, “Unexpected Rule-Changes in a Working Memory Task Shape the Firing of Histologically Identified Delay-Tuned Neurons in the Prefrontal Cortex”
https://www.sciencedirect.com/science/article/pii/S221112471931767X#sec4.1