2 User Reviews

  • Used for IF on primary myocytes and murine cell lines 1:200. Gives nice punctate staining (but lack of negative controls is a problem).

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I have used this antibody for precipitation (IP) of the human insulin receptor tranfected into HEK293 cells. Works very good. I coated the the beads with 1 ug/75 ul beads = 1 IP.

    It also works very well in western blots for detection of the insulin receptor and the proform in both human tissue and murine tissue. Dilution 1:1000.

    I have also used it for immunostaing in hippocampal neurons (1:250), and it gives a believable staining.

    Western blot55555
    Immunostaining44444
    IP55555
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex