This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 23, 2017Christina Demuth wrote:
5
This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines.
Dilution: 1:500
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 16, 2015Jakob Vejby Larsen wrote:
5
I have used this antibody in 293 cells. It works great! Dilution 1:1000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On December 30, 2011Simon Glerup, Aarhus wrote:
4
Great antibody for Western blotting.
It also works for reprobing stripped membranes.
On May 5, 2020 Margie Sutton, University of Texas MD Anderson Cancer Center wrote:
WB dilution 1:2000
IF rating: 5 stars
IHC rating: 4 stars
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5
On September 12, 2017 Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
On January 23, 2017 Christina Demuth wrote:
This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines.
Dilution: 1:500
On January 16, 2015 Jakob Vejby Larsen wrote:
I have used this antibody in 293 cells. It works great! Dilution 1:1000
On December 30, 2011 Simon Glerup, Aarhus wrote:
Great antibody for Western blotting.
It also works for reprobing stripped membranes.
Western blotting dilution: 1:1000