We used this antibody for detection of Actin by Western blotting in C. elegans lysates.
Working dilution: 1:1000 in TBST containing 5% (w/v) milk powder
It works very well.
reference of our publication using the antibody:
Janssens, G.E., Lin, X.X., Millan-Arino, L., Sen, I., Kavsek, A., Seinstra, R.I., Stroustrup, N., Nollen, E.A.A., Riedel, C.G. (2019) Transcriptomics-based screening identifies pharmacological inhibition of Hsp90 as a means to defer aging. Cell Reports 27: 467-480.
On January 3, 2020 Christian Riedel wrote:
We used this antibody for detection of Actin by Western blotting in C. elegans lysates.
Working dilution: 1:1000 in TBST containing 5% (w/v) milk powder
It works very well.
reference of our publication using the antibody:
Janssens, G.E., Lin, X.X., Millan-Arino, L., Sen, I., Kavsek, A., Seinstra, R.I., Stroustrup, N., Nollen, E.A.A., Riedel, C.G. (2019) Transcriptomics-based screening identifies pharmacological inhibition of Hsp90 as a means to defer aging. Cell Reports 27: 467-480.