On December 4, 2019Takahito Higashi, The University of Tokyo wrote:
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I have used this antibody for western blotting of cell lysates from a primary hippocampal neuron culture in a dillution 1 :1000-3000. This antibody works to detect BMP4 prodomain (proBMP4: 45-50kDa). However, I couldn’t detect BMP4 mature domain (~20kDa).
This antibody did not work for immunofluorescence.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
On December 4, 2019 Takahito Higashi, The University of Tokyo wrote:
I have used this antibody for western blotting of cell lysates from a primary hippocampal neuron culture in a dillution 1 :1000-3000. This antibody works to detect BMP4 prodomain (proBMP4: 45-50kDa). However, I couldn’t detect BMP4 mature domain (~20kDa).
This antibody did not work for immunofluorescence.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).