Works very well for immunofluorescence in mouse brain sections. Brains were perfused and post-fixed overnight with 4% paraformaldehyde. Sections were 50-80 μm thick and permeabilized for 30-60 minutes.
Dilution used: 1:2000
For more information see Foggetti et al., Spiny and Non-spiny Parvalbumin-Positive Hippocampal Interneurons Show Different Plastic Properties, Cell Reports, vol 27, issue 13, p3725-3732, June 25, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30737-5).
On May 13, 2020 Dimitri Traenkner, University of Utah wrote:
This antibody works great for IHC.
For more information see Tränkner et al. Cell Reports 2019, “A Microglia Sublineage Protects from Sex-Linked Anxiety Symptoms and Obsessive Compulsion”
https://www.sciencedirect.com/science/article/pii/S2211124719312331
On October 29, 2019 Angelica Foggetti, Christian-Albrechts-Universität zu Kiel wrote:
Works very well for immunofluorescence in mouse brain sections. Brains were perfused and post-fixed overnight with 4% paraformaldehyde. Sections were 50-80 μm thick and permeabilized for 30-60 minutes.
Dilution used: 1:2000
For more information see Foggetti et al., Spiny and Non-spiny Parvalbumin-Positive Hippocampal Interneurons Show Different Plastic Properties, Cell Reports, vol 27, issue 13, p3725-3732, June 25, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30737-5).