This antibody worked fine for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:1000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
On October 17, 2019 David I. Hughes, University of Glasgow wrote:
This antibody worked fine for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:1000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes